Microbiological evaluation of protein quality with Tetrahymena pyriformis W

Ribosome Synthesis in Tetrahymena Pyriformis

The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...

Studies of Membrane Formation in Tetrahymena Pyriformis

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...

Isolation of ceramide-monomethylaminoethylphosphonate from the lipids of Tetrahymena pyriformis W.

Ceramide-monomethylaminoethylphosphonate has been isolated for the first time from the lipids of Tetrahymena pyriformis W and characterized on the basis of its chromatographic mobility, chemical analysis, and infrared and nuclear magnetic resonance properties.

Reactivation of Glycerinated Cilia from Tetrahymena Pyriformis

The ease with which Tetrahymena can be grown in relatively large quantity makes it an exceptionally useful source of cilia for biochemical study. Several methods of isolating the cilia have been described (3, 11). Although excellent for many purposes, these procedures all suffer from the limitation that the isolated cilia seem incapable of being reactivated by ATP. This paper describes a method...

The bioassay of rubratoxins A and B using Tetrahymena pyriformis strain W.